Description
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
Intended Uses: This P38MAPK ELISA kit is intended for Laboratory research use only and is not for use in diagnostic or therapeutic procedures.The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of P38MAPK in the sample, this P38MAPK ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus P38MAPK concentration. The concentration of P38MAPK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This P38MAPK enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a multiclonal antibody specific for P38MAPK. Standards or samples are then added to the microtiter plate wells and P38MAPK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of P38MAPK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for P38MAPK are added to each well to "sandwich" the P38MAPK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain P38MAPK and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm